Protein Expression & Purification
Research Services Beyond All Others
Molecular Throughput, Inc. | 1425 Russ Blvd - Suite T107D | San Diego, CA 92101 | Tel 858.431.9548 | Fax 619.237.1852
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Protein Expression
858.431.9548
Rapid Expression Screening with turnaround time of 2-3 days with multiple parameters tested.

Quantitative Expression Screening - densitometry of SDS-gels to determine mg protein per g cell paste.

Shake Flask Fermentations up to 100L per week.

Isotopic Labeling with 15N, 13C and Selenomethionine.

Periplasmic/Secreted - osmotic shock fluid preparation.
E. coli
Recombinant Baculovirus Generation with expression verification in 21 days.

Sf9, Sf21 and T.ni cells.

BIIC production - baculovirus infected insect cells.

Virus Titer determinations.

Biomass (cell paste) production up to 100L / week.

Secreted Proteins dialyzed and concentrated from media.

Selenomethionine Labeling.
Baculovirus
Mammalian
Transient Transfection using HEK293 and CHO cell lines.

Small-Scale Quantitative Expression Analysis - 30mL cultures with sampling & analysis out to 8 days post-transfection.

Scale up and Biomass Production up to 50L per week.

Secreted Proteins stabilization and diafiltration of culture supernatants.
Call us with your specific protein expression requirements!